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SOLUBLE HEMIN COMPOUNDS AS ULTRASTRUCTURAL TRACERS

J. F. ARONSON 1, A. P. FISHMAN 1, and G. G. PIETRA 2

1 Cardiovascular-Pulmonary Division, Department of Medicine, University of Pennsylvania, Philadelpha, Pennsylvania 19104
2 Cardiovascular-Pulmonary Division, Department of Medicine and Department of Pathology, University of Pennsylvania, Philadelpha, Pennsylvania 19104

We have demonstrated that soluble hemin derivatives have electron microscope-detectable peroxidase activity. Hemin was coupled to several different size classes of dextran, to sucrose and to glucuronic acid. The peroxidase activity of the attached hemin was detected with the electron microscope using the method described by Graham and Karnovsky for horseradish peroxidase. These observations indicate that hemin may be used as a prosthetic group to label macromolecules for electron microscopic localization and raise the prospect of creating groups of inexpensive tracer substances of varying size, charge and specificity for histologic and ultrastructural studies.

Submitted on May 23, 1973


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