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KINETICS OF HYDROLYSIS DURING THE FEULGEN REACTION FOR DEOXYRIBONUCLEIC ACID A REEVALUATION

ROBERT W. RASCH 1 and ELLEN M. RASCH 2

1 Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53233
2 Department of Biology, Marquette University, Milwaukee, Wisconsin 53233

Using thin films of Feulgen-stained chicken blood cells, we have found that the course of deoxyribonucleic acid (DNA) hydrolysis with 5 N HCl at room temperature over a period of 24 hr can be represented by equations based upon a simple kinetic model involving branched convergent reactions. In addition to its use for analysis of the Feulgen hydrolysis curves generated from avian erythrocytes and lymphocytes, the model has also been applied to previously published data on human lymphocytes. Use of the model to determine appropriate conditions for validating stoichiometry of the DNA-Feulgen reaction is discussed, with particular emphasis directed to possible implications for DNA-Feulgen cytophotometry of a two-compartment substrate moiety.

Submitted on June 6, 1973


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