Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PRATT, R. M.
Right arrow Articles by GIBSON, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PRATT, R. M., JR.
Right arrow Articles by GIBSON, W. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

AN IMMUNOFLUORESCENT TECHNIQUE FOR OBSERVING THE BINDING OF CONCANAVALIN A TO FROZEN TISSUE SECTIONS

ROBERT M. PRATT JR. 1 and WILLIAM A. GIBSON 1

1 National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

An indirect fluorescent antibody technique has been developed to observe the binding locus of concanavalin A (CON A) to specific structures in frozen tissue sections. The technique was developed using frontal sections of the embryonic rat head with particular attention focused on the secondary palate. The binding of CON A appeared to be mainly extracellular with the most intense fluorescence observed in basement lamina and cartilaginous structures. CON A binding was highly specific in that it was eliminated in the presence of competing sugars such as agr-methyl mannoside. This relatively quick and simple technique should allow the observation of developmental or pathologic changes in CON A binding to frozen tissue sections from a variety of sources.

Submitted on October 10, 1972


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1973