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PREPARATION AND USE OF SOLUBLE FERRITIN-ANTIFERRITIN COMPLEXES AS A SPECIFIC MARKER FOR IMMUNOELECTRON MICROSCOPY

AMERICO A. MARUCCI 1, ROBERT M. DOUGHERTY 1, and HENRY S. DISTEFANO 2

1 Departments of Microbiology State University of New York, Upstate Medical Center, Syracuse, New York 13210
2 Department of Anatomy, State University of New York, Upstate Medical Center, Syracuse, New York 13210

Soluble immune complexes of ferritin and chicken antiferritin have been prepared. This has been used with unlabeled chicken antibody to avian leukosis virus and rabbit antichicken globulin to demonstrate the presence of specific viral antigens in tissue culture by electron microscopy.

Submitted on May 23, 1973


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