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PEROXIDASE-LABELED ANTIBODY A NEW METHOD OF CONJUGATION
1 Department of Pathology, University of Colorado Medical Center, Denver, Colorado 80220
A new method of conjugating horseradish peroxidase with proteins was developed. The carbohydrate moiety of fluorodinitrobenzene-blocked peroxidase was oxidized with sodium periodate to form aldehyde groups. The peroxidase-aldehyde was then bound to free amino groups of proteins unidirectionally at high efficiencies. Peroxidase-labeled immunoglobulin retained its immunologic as well as enzymatic activities.
Submitted on May 29, 1974Revised on August 31, 1974
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