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DEMONSTRATION OF HISTONASE WITH FLAVIANIC ACID

LAWRENCE KASS 1

1 Department of Internal Medicine (Simpson Memorial Institute), The Unversity of Michigan Medical School, Ann Arbor, Michigan 48104

A method was developed to demonstrate histonase activity in the cytoplasm of polymorphonuclear leukocytes, eosinophils and monocytes. The technique was based on the ability of flavianic acid (2,4-dinitro-l-naphthol-7-sulfonic acid) to form highly insoluble salts with arginine. Presumably, the source of the free arginine was from histone proteolysis as a result of the action of histonase.

Submitted on April 1, 1974
Revised on July 5, 1974


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