Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by YAROM, R.
Right arrow Articles by CHANDLER, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by YAROM, R.
Right arrow Articles by CHANDLER, J. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

ELECTRON PROBE MICROANALYSIS OF SKELETAL MUSCLE

R. YAROM 1 and J. A. CHANDLER 2

1 Department of Pathology, Hebrew University-Hadassah Medical School, Jerusalem, Israel
2 Tenovus Institute for Cancer Research, Welsh National School of Medicine, Heath, Cardiff, United Kingdom

X-ray microanalysis using the analytical electron microscope (EMMA 4) was performed on resting frog sartorius muscles fixed by the pyroantimonate technique. Calcium was the main element of interest analyzed. Pretreatment was with ethyleneglycoltetraacetate to chelate calcium and with Tris chloride to replace sodium. Osmium-fixed controls were also analyzed. Both crystal-diffracting and energy-dispersive analyzers were employed, the former for accurate quantitation of calcium content and the latter for qualitative assessment of other elements present. Significant concentrations of calcium occurred in the dense parts of the nucleus, the triads, the thin filaments and the sarcolemma. The amount of calcium was greatly decreased by ethyleneglycoltetraacetate pretreatment and slightly increased by sodium removal. After osmium fixation alone, little calcium was detected with no localization.

Submitted on June 15, 1973


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1974