Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by KOELLE, G. B.
Right arrow Articles by KOELLE, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by KOELLE, G. B.
Right arrow Articles by KOELLE, W. A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

EFFECTS OF ALDEHYDE FIXATION AND OF PREGANGLIONIC DENERVATION ON ACETYLCHOLINESTERASE AND BUTYROCHOLINESTERASE OF CAT AUTONOMIC GANGLIA

GEORGE B. KOELLE 1, RICHARD DAVIS 1, and WINIFRED A. KOELLE 1

1 Department of Pharmacology, Medical School, University of Pennsylvania, Philadelphia, Pennsylvania 19174

Fixation of cat stellate ganglia by perfusion in situ with cold 4% formaldehyde in Krebs-Ringer solution, followed by immersion in the same solution for 3 or 6 hr, produced inactivation of approximately one-half the acetylcholinesterase (AChE) and butyrocholinesterase (BuChE); after 21 hr, further inactivation occurred. Similar fixation with glutaraldehyde (1, 2 and 4%) inactivated over three-quarters of the AChE and BuChE at 3 hr, but there was little further loss after 21 hr. Following sectioning of the preganglionic trunk, the AChE activity of the cat superior cervical ganglion fell to approximately 20%, and BuChE fell to approximately 50%; this confirms an early report by Sawyer and Hollinshead, which has been questioned in part because of subsequent histochemical findings. The basis for the discrepancy is discussed, along with its bearing on the interpretation of histochemical observations in general.

Submitted on August 6, 1973


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1974