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SPECTRAL MATCHING OF CLASSICAL CYTOCHEMISTRY TO AUTOMATED CYTOLOGY

LEONARD ORNSTEIN 1 and HUDSON R. ANSLEY 2

1 Cell Research Laboratory, Department of Pathology, Mount Sinai School of Medicine of the City University of New York, New York, New York 10029
2 Automation Development Laboratory, Mount Sinai School of Medicine of the City University of New York, New York, New York 10029

Aspects of the history of automated cytology and a brief comparison of present and anticipated future levels of performance of flow-through and pattern recognition technologies for white blood cell recognition and counting are briefly presented. The relationship of the spectral properties of cytochemical preparations to machine performance are discussed. Some advantages of matching the spectral properties to hardware and software capabilities are illustrated. Some kinds of difficulties in achieving such a match are reviewed and examples of present levels of achievement are illustrated.

Submitted on February 6, 1974


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