Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by GILL, J. E.
Right arrow Articles by JOTZ, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by GILL, J. E.
Right arrow Articles by JOTZ, M. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

DEOXYRIBONUCLEIC ACID CYTOCHEMISTRY FOR AUTOMATED CYTOLOGY

J. E. GILL 1 and M. M. JOTZ 1

1 Bio-Medical Divison, Lawrence Livermore Laboratory, University of California, Livermore, California 94550

Fluorescent staining for intracellular deoxyribonucleic acid content is being investigated as a means of making automated measurements quantitative and reliable. Ambiguities regarding the role of sulfur dioxide in Feulgen staining chemistry have been resolved showing that: (a) SO2 does not covalently bind to the anilinium moieties of pararosaniline; (b) SO2 does promote the binding of pararosaniline within the nuclei of hydrolyzed cells; (c) SO2 also does not bind to dyes such as acriflavine used in fluorescent-"Feulgen" staining and (d) SO2 is not required for strong, nucleus-specific acriflavine staining of nuclei of hydrolyzed cells. These findings permit simplifications of the usual fluorescent-"Feulgen" procedures. Attempts to use fluorescent intercalating dyes as vital stains for deoxyribonucleic acid content have been unsuccessful.

Submitted on March 20, 1974


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 ScienceHome page
P. Horan and L. Wheeless Jr
Quantitative single cell analysis and sorting
Science, October 14, 1977; 198(4313): 149 - 157.
[PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1974