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COLOR INFORMATION IN BLOOD CELLS

GEORGE C. CHENG 1

1 Center for Informatics Research, University of Florida, Gainesville, Florida 32611

Two hundred color spectra of blood cells treated with Wright-Giemsa stain were measured by using an Olympus microspectrophotometer. The wavelength of the light source was varied from 400 to 700 nm. Several basic pictorial regions in the blood smear were investigated. These regions included the background, basophil, eosinophil, neutrophil, lymphocyte, monocyte and the red blood cell. A subset of the color spectrum curves was used as references. The remaining spectrum curves were used as testing samples and were shown to individuals who had no prior knowledge of leukocyte color information. All of the persons tested could correctly identify the testing samples with the reference samples. This experiment showed that color information in blood cells is not only useful but also sufficient for leukocyte differentiation for the samples that have been investigated.

Submitted on January 14, 1974


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