Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by MENDELSOHN, M. L.
Right arrow Articles by MAYALL, B. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by MENDELSOHN, M. L.
Right arrow Articles by MAYALL, B. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

COMPUTER-ORIENTED ANALYSIS OF HUMAN CHROMOSOMES IV. DEOXYRIBONUCLEIC ACID-BASED CENTROMERIC INDEX

MORTIMER L. MENDELSOHN 1, DEBORAH E. BENNETT 1, ELLIOT BOGART 1, and BRIAN H. MAYALL 1

1 Biomedical and Environmental Research Division, Lawrence Livermore Laboratory, University of California, Livermore, California 94550

A density-oriented semiautomatic method to estimate centromeric index is described and tested on 4611 human chromosomal images. Chromosomes are stained for deoxyribonucleic acid with gallocyanin-chrome alum and their optical density is recorded by a digital scanning microscope. Shape information in the chromosome boundary leads to a provisional centromeric position. The chromosome is divided into strips 0.25-0.35 µm wide paralleling the centromere. Optical density is integrated within each strip, strips are assembled into a density profile and the definitive centromere is located at the major local minimum in the profile. Centromeric index (large arm:total) is based on optical density or area. A test set of 4611 chromosomal images is based on over 2500 chromosomes (56 metaphase cells from six individuals) and excludes overlapped and excessively bent chromosomes. The program fails in 1.2% of the test images. Standard deviation within chromosome groups of centromeric index based on density is 0.024 and replication error is 0.016. Systematic differences between area-based and density-based measurements are observed and interpreted.

Submitted on April 24, 1974


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1974