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VISUALIZATION OF DEOXYRIBONUCLEIC ACID MOLECULES BY PROTEIN FILM ADSORPTION AND TANTALUM-TUNGSTEN SHADOWING

REINHARD ABERMANN 1 and MIRIAM M. SALPETER 1

1 School of Applied and Engineering Physics, Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14850

Preparatory procedures and high resolution shadowing of deoxyribonucleic acid (DNA) molecules for electron microscopy are described. They involve a two-stage mounting of the DNA on cytochrome c monolayers and shadowing with tantalum-tungsten. Conditions are determined that produce a smooth substrate and reproducible binding of the DNA strands in a randomly coiled manner without obvious stretching artifacts. When limiting factors such as excess buffer salts are eliminated, the DNA strands have the over-all dimensions expected from the naked molecule.

Submitted on November 12, 1973


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