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Bismuth localization within nuclear inclusions by x-ray microanalysis. Effects of accelerating voltage

BA Fowler and RA Goyer

This report details the localization of bismuth by energy-dispersive x- ray microanalysis within characteristic nuclear inclusion bodies of renal tubular lining cells of rats following excessive exposure to this element. Peak to background ratios and analytical detection sensitivities for bismuth were found to vary for 04, 60 or 80 keV electron accelerating voltages. Optimum peak to background ratios were observed at 40 keV due to lower background generation, while greater detection sensitivities were recorded at 80 keV due to enhanced generation of bismuth characteristic x-rays.

Volume 23, Issue 10, pp. 722-726, 10/01/1975
Copyright © 1975 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1975