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Rapid chemical dehydration of samples for electron microscopic examinations

LL Muller and TJ Jacks

Acidified 2,2-dimethoxypropane was used to chemically dehydrate biologic tissues for examination in the electron microscope. The ultrastructural integrities of single-celled algae, plant tissues (cotyledon, root, leaf) and animal tissues (liver, pancreas, muscle, cartilage) were maintained. Our technique was simpler and quicker than physically exchanging water for organic solvents (e.g., acetone, ethanol) as generally performed in microscopy.

Volume 23, Issue 2, pp. 107-110, 02/01/1975
Copyright © 1975 by The Histochemical Society


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 Textile Research JournalHome page
K. Rajasekaran, A. J. Muir, B. F. Ingber, and A. D. French
A Dehydration Method for Immature or Wet Cotton Fibers for Light and Electron Microscopy
Textile Research Journal, June 1, 2006; 76(6): 514 - 518.
[Abstract] [PDF]



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