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Application of immunoperoxidase techniques to localize horseradish peroxidase-tracer in the central nervous system

LL Vacca, SL Rosario, EA Zimmerman, P Tomashefsky, NG Po-Ying and KC Hsu

Immunoperoxidase techniques are presented which can be used to localize horseradish peroxidase-tracer in paraffin-embedded tissues of the central nervous system. Compared to histochemical methods using frozen sections, these immunologic techniques allow the use of stored, serial paraffin sections, and appear more sensitive for the demonstration of intraneuronal horseradish peroxidase after retrograde transport. The immunoperoxidase bridge techniques from reaction products of high quality which can easily be seen in fine processes.

Volume 23, Issue 3, pp. 208-215, 03/01/1975
Copyright © 1975 by The Histochemical Society


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