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The efficiency of 14C detection in autoradiographs of semithin plastic sections

TL Housley and DB Fisher

Sections of uniformly labeled 14C-methylmethacrylate were used to determine the efficiency of 14C detection by Ilford L4 emulsion layers, 0.22 mu in thickness, in light microscope autoradiographs. When efficiency was expressed as a function of radiation dose (i.e., the number of decays/mu2) there was a strong dependency on radiation dose up to about 6 decays/mu2, after which detection efficiency was fairly constant. For most emulsion batches, the detection efficiency was about 5%, but it ranged from 8 to 3%.

Volume 23, Issue 9, pp. 678-680, 09/01/1975
Copyright © 1975 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1975