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Cell electrophoresis research directed toward clinical cytodiagnosis

RC Boltz , P Todd, RA Gaines, RP Milito, JJ Docherty, CJ Thompson, MF Notter, LS Richardson and R Mortel

The application of cell electrophoresis to cytodiagnosis requires that a scientifically established basis exists for identifying abnormal cells electrophoretically, that research to detect such differences in the cytodiagnostic setting is possible and that a rapid and simple method of cell electrophoresis is adaptable to the clinical setting. Data are presented indicating modifications of electrophoretic mobility due to herpes simplex virus type 1 infection and Rous sarcoma virus transformation of culture cells. A simple apparatus for electrophoretically separating cells on a density gradient and collecting them for subsequent analysis is described, and results of experiments with this apparatus are consistent with those obtained by microscopic electrophoresis. Laser-doppler spectroscopic electrophoresis is suggested as a rapid method adaptable to clinical application.

Volume 24, Issue 1, pp. 16-23, 01/01/1976
Copyright © 1976 by The Histochemical Society


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