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Flow microfluorometric system for screening gynecologic cytology specimens using propidium iodide-fluorescein isothiocyanate

BJ Fowlkes, CJ Herman and M Cassidy

Seventy cervical cytology specimens have been screened by a xero resolution flow analyzer-sorter using propidium iodide and fluorescein isothiocyanate as fluorochromes for nucleus and cytoplasm, respectively. This system shows a 1% sensitivity for detection of abnormal cells using only crude visual data analysis. Screening of clinical specimens was performed on the instrument with a 5.8% false negative rate and a 11.8% false positive rate by comparison with routine visual cytologic evaluation of the same samples.

Volume 24, Issue 1, pp. 322-331, 01/01/1976
Copyright © 1976 by The Histochemical Society


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P. Horan and L. Wheeless Jr
Quantitative single cell analysis and sorting
Science, October 14, 1977; 198(4313): 149 - 157.
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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1976