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Plasma protein immunocytochemistry and polysaccharide cytochemistry at the surface of alveolar and endothelial cells in the rat lung

J Bignon, F Jaubert and MC Jaurand

Ultrastructural immunocytochemistry of plasma proteins and cytochemistry of polysaccharides with ruthenium red and concanavalin A were combined with different types of fixation with or without prior vascular or airway washing, to study the surface of capillary endothelial and alveolar epithelial cells of the blood-air barrier in the rat lung. The endothelial and epithelial cell surface layers were found to have two components: a moveable part belonging to the cellular microenvironement made of plasma proteins and a deeper glucidic or anionic fixed part bound to the plasma membranes (cell coat).

Volume 24, Issue 10, pp. 1076-1084, 10/01/1976
Copyright © 1976 by The Histochemical Society


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