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A microspectrophotometric study of Papanicolaou-stained cervical cells as an aid in computerized image processing

J Holmquist, Y Imasoto, E Bengtsson, B Olsen and B Stenkvist

As part of a study of cytologic automation, microspectrophotometric investigation of Papanicolaou-stained cervical cells was performed, using a Leitz MPV-II scanning photometer connected to a PDP 8/F minicomputer. It was shown that the selection of one single wavelength may result in difficulties in detecting boundries between background and cytoplasm and/or between cytoplasm nucleus. A set of two wavelengths, 530 nm and 570 nm, were found to be optimal for the image processing of these cells.

Volume 24, Issue 12, pp. 1218-1224, 12/01/1976
Copyright © 1976 by The Histochemical Society


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