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Immunoelectronmicroscopic localization of alpha-actinin in skeletal muscle cells

BP Lane, J Elias and E Drummond

The ultrastructural distribution of alpha-actinin in skeletal myocytes was studied by a sensitive method that permits localization of antigenic proteins in intact fixed cells rather than in glycerol estracted tissues or isolated cell fractions. Binding to the Z line of specific antiserum produced in rabbits immunized with highly purified alpha-actinin was demonstrated by deposition at this site of a reaction product of horseradish peroxidase that had been coupled to antigen- binding fragments of sheep anti-rabbit gamma globulin.

Volume 25, Issue 1, pp. 69-72, 01/01/1977
Copyright © 1977 by The Histochemical Society


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