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Purification of commercial acriflavine by Sephadex LH-20 column chromatography

JW Levinson, VM Maher and JJ McCormick

Acriflavine supplies commercially was purified by removing contaminating proflavine by chromatography on a column of Sephadex LH- 20 eluded with 65% methanol. The purified acriflavine, recovered in a yield that was 52% of the theoretical maximum, had a visible absorption maximum of 452 nm with a band width of 47 nm. These spectral properties agreed with those of acriflavine purified by precipitation, repeated recrystallization and chromatography on aluminum oxide, which results in a yield of 13% at best.

Volume 25, Issue 11, pp. 1275-1277, 11/01/1977
Copyright © 1977 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1977