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A technique for the immunohistochemical localization of prostaglandin E

DE Van Orden, JM Klemm and JA Redick

Prostaglandin E (PGE) has been localized via the unlabeled antibody technique in freeze-dried and ethanol-fixed cryostat sections. Discrete perivascular and stromal localization was present in the uterus prepared by the method presented, but not in classically fixed specimens. Absorption of the anti-PGE by addition of free PGE was ineffective; whereas, removal of PGE-reactive antibodies from the anti- serum was effectively accomplished with an Affigel-101-PGE immunoadsorbant column.

Volume 25, Issue 12, pp. 1291-1296, 12/01/1977
Copyright © 1977 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1977