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Variable opacity of glycogen in routine electron micrographs

LE Thornell, M Sjostrom, U Karlsson and E Cedergren

Glycogen in nerve terminals from the reticular zone of frog muscle was identified by transmission electron microscopy of both periodic acid- thiosemicarbazide-silverproteinate treated and UAc-PbCi-stained serial sections. A variable appearance of glycogen in the uranylacetate-lead citrate-stained nerve terminals was seen and is related to the preparative procedure. The study indicates the necessity of cytochemical identification for the assessment of glycogen organization in cells.

Volume 25, Issue 9, pp. 1069-1073, 09/01/1977
Copyright © 1977 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1977