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Device for quantitative cytochemistry: a computerized scanning microdensitometer 'grain counter'

NS Markovic, LE Lipkin, OS Markovic and MJ Wade

A computerized scanning microdensitometer and autoradiographic grain counter was able to provide quantitative data on the cytochemical final reaction product formed within a single cell and also quantitate the kinetics of its formation. Optical density and area measurements were performed on hundreds of leukocytes from slides previously stained to demonstrate any one of a variety of reactions. These included cellular glycogen, lipids, peroxidase, esterases, alkaline phosphatase, and acid phosphatase. In addition to these slide studies, chamber studies with an adapted Dvorak-Stotler Chamber allowed the measurement of enzyme kinetics within single cells.

Volume 26, Issue 10, pp. 792-802, 10/01/1978
Copyright © 1978 by The Histochemical Society


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