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A comparison of mercury arc lamp and laser illumination for flow cytometers

DC Peters

Optical differences between a mercury arc lamp and a laser-illuminated flow cytometer are compared. The distributions of spectral intensities of the two light sources are shown in relation to the excitation characteristics of the fluorescent dyes acriflavine, chromomycin A3, mithramycin, ethidium bromide, Hoechst 33258, and 4,6-diamidino-2- phenylindole (DAPI). Fluorescence intensities of microspheres and Hoechst 33258-stained mouse sperm are compared in the two cytometers. The optical efficiencies are similar and depend on the match of the excitation characteristics of the stain with the emission spectra of the light source.

Volume 27, Issue 1, pp. 241-245, 01/01/1979
Copyright © 1979 by The Histochemical Society


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 Clin. Chem.Home page
F. Ferrara, R. Daverio, G. Mazzini, P. Bonini, and G. Banfi
Automation of human sperm cell analysis by flow cytometry
Clin. Chem., May 1, 1997; 43(5): 801 - 807.
[Abstract] [Full Text] [PDF]



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