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Scintillation fluid shortens exposure times in autoradiography

BM Stanulis, S Sheldon, GL Grove and VJ Cristofalo

Premixed, commercially available scintillation fluids were used to reduce exposure time of tritium (3H) and iodine-125 (125I)-labeled whole cells, and of 3H-labeled Giemsa-banded chromosome preparations. Emulsion-coated slides were dipped into scintillator for no longer than 2 min and exposed in the dark at 4 degrees C. Maximal values for percentage of human diploid cell (WI-38) nuclei labeled with 3H- thymidine of moderate specific activity were obtained in 12 hr. Without scintillator the exposure time was 4 days. Exposure time for cells labeled with 125I-serum was reduced from over 90 days to 14 days. The shortened exposure time for banded chromosomes permitted successful prestaining with Giemsa, a sequence that is not possible without scintillator.

Volume 27, Issue 10, pp. 1303-1307, 10/01/1979
Copyright © 1979 by The Histochemical Society


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B Gazit, H Cedar, I Lerer, and R Voss
Active genes are sensitive to deoxyribonuclease I during metaphase
Science, August 13, 1982; 217(4560): 648 - 650.
[Abstract] [PDF]



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