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Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers

MS Ghandour, OK Langley, G Vincendon and G Gombos

Sequential treatment of rat cerebellar slices with two antisera, each specific for a different glial cell marker, revealed, by indirect immunofluroescence combined with the immunoperoxidase method on the same tissue section, that carbonic anhydrase isoenzyme II (CA II) is exclusively localized in oligodendrocytes while the glial fibrillary acidic protein (GFA) is, as well established, a specific marker for astrocytes.

Volume 27, Issue 12, pp. 1634-1637, 12/01/1979
Copyright © 1979 by The Histochemical Society


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