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Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry

A Krishan and R Ganapathi

The intracellular distribution of important chemotherapeutic antibiotics belonging to the anthracycline group (e.g. adriamycin) can be detected by laser flow cytometry. The indirect method is based on the interference of these compounds with the binding of propidium iodide to the nuclear DNA. While in the direct method, the intracellular fluorescence of these antibiotics is excited and detected with a laser beam in a flow system. The present report demonstrates the use of these two methods for intracellular detection and quantitation of a number of important anthracyclines.

Volume 27, Issue 12, pp. 1655-1656, 12/01/1979
Copyright © 1979 by The Histochemical Society


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