The use of the potassium pyroantimonate-osmium method as a means of identifying and localizing calcium at the ultrastructural level in the cells of calcifying systemsJ Appleton and DC Morris
Potassium pyroantimonate-osmium has been used to localize calcium as an electron dense precipitate in the odontoblast, ameloblast and early hypertrophic chondrocytes of the mandibular condylar growth cartilage. The precipitate exhibited a specific association with various cell organelles. The presence of calcium in the precipitates was confirmed using energy dispersive analysis by x-rays. It is suggested that this K- pyroantimonate method provides an accurate technique for the investigation of the subcellular localization of calcium in calcifying systems.
Volume 27,
Issue 2,
pp. 676-680,
02/01/1979
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H.-G. Lu, L. Zhong, W. de Souza, M. Benchimol, S. Moreno, and R. Docampo Ca2+ Content and Expression of an Acidocalcisomal Calcium Pump Are Elevated in Intracellular Forms of Trypanosoma cruzi Mol. Cell. Biol., April 1, 1998; 18(4): 2309 - 2323. [Abstract] [Full Text] |
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