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The use of avidin-biotin interaction in immunoenzymatic techniques

JL Guesdon, T Ternynck and S Avrameas

Biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. Based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme- immunoassay and enzyme-immunohistochemical staining procedures. Two procedures were developed. In the first procedure, named the Bridged Avidin-Biotin (BRAB) technique four steps were used sequentially in order to quantify or detect an immobilized antigen: 1) incubation with biotin-labeled antibody; 2) incubation with avidin; 3) incubation with biotin-labeled enzyme; 4) measurement or histochemical staining of the enzyme. The technique is based on the observation that avidin possesses four active sites. In the second procedure, named the Labeled Avidin- Biotin (LAB) technique, biotin-labeled antibody and enzyme-labeled avidin are used sequentially. Enzyme-associated antigen is then quantified or revealed immunohistochemically. The optimal conditions for enzyme-immunoassay and enzyme-immunohistochemical staining using BRAB and LAB procedures were established.

Volume 27, Issue 8, pp. 1131-1139, 08/01/1979
Copyright © 1979 by The Histochemical Society


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 J. Neurosci.Home page
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 J. Nutr.Home page
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[Abstract] [Full Text] [PDF]

Home page
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Home page
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[Abstract] [Full Text] [PDF]

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[Abstract] [Full Text]

Home page
 J. Am. Soc. Nephrol.Home page
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[Abstract] [Full Text]

Home page
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Effects of neurotransmitters, gut hormones, and inflammatory mediators on mucus discharge in rat colon
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[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Ni, M. A. Fernandez, L. Danielsson, R. A. Chillakuru, J. Zhang, A. Grubb, J. Su, R. Gentz, and M. Abrahamson
Cystatin F Is a Glycosylated Human Low Molecular Weight Cysteine Proteinase Inhibitor
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[Abstract] [Full Text] [PDF]

Home page
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[Abstract] [PDF]

Home page
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Home page
 BloodHome page
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[Abstract] [Full Text] [PDF]

Home page
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[Abstract] [Full Text] [PDF]

Home page
 Br. J. Ophthalmol.Home page
A. J E Foss, R. A Alexander, J. L Hungerford, A. L Harris, I. A Cree, and S. Lightman
Reassessment of the PAS patterns in uveal melanoma
Br. J. Ophthalmol., March 1, 1997; 81(3): 240 - 246.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
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Immunocytochemical Phenotyping of Disseminated Tumor Cells in Bone Marrow by uPA Receptor and CK18: Investigation of Sensitivity and Specificity of an Immunogold/Alkaline Phosphatase Double Staining Protocol
J. Histochem. Cytochem., February 1, 1997; 45(2): 203 - 212.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
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Biotin and Digoxigenin as Labels for Light and Electron Microscopy in Situ Hybridization Probes: Where Do We Stand?
J. Histochem. Cytochem., February 1, 1997; 45(4): 481 - 492.
[Abstract] [Full Text] [PDF]

Home page
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Inhibition of gastric acid secretion by stress: A protective reflex mediated by cerebral nitric oxide
PNAS, December 10, 1996; 93(25): 14839 - 14844.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L.-M. Chen, J.-x. Ma, Y.-M. Liang, L. Chao, and J. Chao
Tissue Kallikrein-binding Protein Reduces Blood Pressure in Transgenic Mice
J. Biol. Chem., November 1, 1996; 271(44): 27590 - 27594.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kobayashi, J. Gotoh, Y. Hirashima, and T. Terao
Inter-alpha-trypsin Inhibitor Bound to Tumor Cells Is Cleaved into the Heavy Chains and the Light Chain on the Cell Surface
J. Biol. Chem., May 10, 1996; 271(19): 11362 - 11367.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
W. Xiong, J. Chao, and L. Chao
Muscle Delivery of Human Kallikrein Gene Reduces Blood Pressure in Hypertensive Rats
Hypertension, April 1, 1995; 25(4): 715 - 719.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
J.-x. Ma, Z. Yang, J. Chao, and L. Chao
Intramuscular Delivery of Rat Kallikrein-binding Protein Gene Reverses Hypotension in Transgenic Mice Expressing Human Tissue Kallikrein
J. Biol. Chem., January 6, 1995; 270(1): 451 - 455.
[Abstract] [Full Text] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
U.R. Rapp, G. Heidecker, M. Huleihel, J.L. Cleveland, W.C. Choi, T. Pawson, J.N. Ihle, and W.B. Anderson
raf Family Serine/Threonine Protein Kinases in Mitogen Signal Transduction
Cold Spring Harb Symp Quant Biol, January 1, 1988; 53(0): 173 - 184.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
C. T. Warnick, B. Dabbas, C. D. Ford, and K. A. Strait
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J. Biol. Chem., July 13, 2001; 276(29): 27363 - 27370.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hirono, T. Sugiyama, Y. Kishimoto, I. Sakai, T. Miyazawa, M. Kishio, H. Inoue, K. Nakao, M. Ikeda, S. Kawahara, et al.
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J. Biol. Chem., November 21, 2001; 276(48): 45236 - 45242.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
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