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Trypsin digestion in immunoperoxidase staining

NW Hautzer, JF Wittkuhn and WT Mccaughey

The effect of trypsin digestion on the immunoperoxidase-PAP staining of paraffin sections from normal and abnormal tissue was studied. Trypsin treatment reduced background staining considerably, but enhanced specific staining. Results were, therefore, much easier to read. Possible reasons for this result are discussed.

Volume 28, Issue 1, pp. 52-53, 01/01/1980
Copyright © 1980 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1980