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Evaluating optical filter efficiency in a flow cytometer

MR Loken

A simple yet effective method of testing optical filters under normal operating conditions on a Fluorescence Activated Cell Sorter is described. The spectral characteristics of three filters are compared to demonstrate the methods of determining which filter is the best in a particular situation. These tests include determining the efficiency of transmitting fluorescent light, determining the efficiency of removing scattered light, and detecting fluorescence originating from the filter itself.

Volume 28, Issue 10, pp. 1136-1137, 10/01/1980
Copyright © 1980 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1980