Use of the protein A-gold technique for the morphological study of vascular permeabilityM Bendayan
The protein A-gold immunocytochemical technique has been applied for the ultrastructural study of vascular permeability. A capillary preparation was perfused for 30 min with a buffer solution containing albumin. After fixation and embedding, the albumin molecules were revealed on the tissue sections using anti-albumin antiserum and the protein A-gold complex. A specific labeling was obtained over the capillary lumen, the vesicular structures of the endothelial cells, and the intercapillary space. These results allowed us to conclude that the protein A-gold technique is suitable and of general applicability for the morphological study of vascular permeability.
Volume 28,
Issue 11,
pp. 1251-1254,
11/01/1980
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M. Bendayan and E. A. Rasio Evidence of a Tubular System for Transendothelial Transport in Arterial Capillaries of the Rete Mirabile J. Histochem. Cytochem., October 1, 1997; 45(10): 1365 - 1378. [Abstract] [Full Text] [PDF] |
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M Bendayan and E. Rasio Transport of insulin and albumin by the microvascular endothelium of the rete mirabile J. Cell Sci., January 7, 1996; 109(7): 1857 - 1864. [Abstract] [PDF] |
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