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Determination of immunoreactive melatonin in the colon of the rat by immunocytochemistry

WR Holloway, LJ Grota and GM Brown

Melatonin immunofluorescence in the rat colon was measured with a cadmium sulfide photometer in conjunction with an immunohistological double antibody procedure. The method proved to be very reliable with 5- 10% intra- and interassay reliabilities. In addition, manipulation of the primary antibody concentration, the incubation time, and the nature of the first antibody produced predicted changes in fluorescence intensity. Treatment with p-chlorophenylalanine led to a marked reduction of melatonin-specific fluorescence.

Volume 28, Issue 3, pp. 255-262, 03/01/1980
Copyright © 1980 by The Histochemical Society


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