Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sisson, S. P.
Right arrow Articles by Vernier, R. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sisson, S. P.
Right arrow Articles by Vernier, R. L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Methods for immunoelectron microscopy: Localization of antigens in rat kidney

SP Sisson and RL Vernier

This study evaluated a variety of fixatives and methods of tissue preparation for application of the direct peroxidase-labeled antibody technique to rat kidney specimens. Tissue ultrastructure was most satisfactorily preserved and the antigens studied (rabbit IgG, human IgG, and rat Tamm-Horsfall protein (THP) were adequately preserved after brief fixation with 1% glutaraldehyde (15 min), a mixture of paraformaldehyde (1%) and glutaraldehyde (0.05%), or a paraformaldehyde, lysine, periodate fixative. Glycerol substitution was considered an important step which minimized ice crystal artifacts. Freezing and thawing were essential steps that facilitated adequate penetration of labeled antibody to specific antigenic sites. The distribution of injected rabbit IgG (anti-rat glumorular basement membrane (GBM) antibody) was predominately on the lamina rara interna and externa of the GBM. Injected aggregated human IgG was found primarily within the spaces between glomerular mesangial cells. Rabbit anti-rat THP was localized primarily on the infolding membranes of cells of the ascending thick limb of Henle. We suggest that the methods described may have wide application.

Volume 28, Issue 5, pp. 441-452, 05/01/1980
Copyright © 1980 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1980