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A reliable Feulgen-acriflavine-SO2 staining procedure for quantitative DNA measurements

HJ Tanke and EM van Ingen

Feulgen-acriflavine-SO2 staining was performed on chicken erythrocytes and rat liver cells under different cytochemical conditions. The obtained results were compared to conventional Feulgen-pararosaniline- SO2 staining by means of scanning cytophotometry and microfluorometry. It was found that if acid ethanol rinsing was included in the Feulgen- acriflavine-SO2 procedure, both Feulgen procedures were fully comparable in specificity and quantitative aspects.

Volume 28, Issue 9, pp. 1007-1013, 09/01/1980
Copyright © 1980 by The Histochemical Society


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