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Hemocyanin linked to protein A as an immunochemical labeling reagent for electron microscopy

MM Miller, CD Strader, MA Raftery and JP Revel

Hemocyanin-protein A (Hcy/A) is an immunochemical marker suitable for both transmission and scanning electron microscopy. A method is described for the preparation of highly monomeric Hcy/A where protein A and hemocyanin are present in the conjugate in a nearly equal molar ratio. Examples of Hcy/A labeling of several cellular antigens are provided.

Volume 29, Issue 11, pp. 1322-1327, 11/01/1981
Copyright © 1981 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1981