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Effect of actinomycin-D on the staining of whole cells with acridine orange

SH Chambers and JV Watson

Flow cytofluorometric techniques have been used to investigate the interference of actinomycin-D with the staining of acridine orange in whole cells. The results show that reduction in fluorescence intensity will only occur at actinomycin-D concentrations greater than 10(3) ng/ml in our system, providing the drug is washed out prior to staining.

Volume 29, Issue 5, pp. 644-648, 05/01/1981
Copyright © 1981 by The Histochemical Society


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