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OTO method for preservation of actin filaments in electron microscopy

M Aoki and M Tavassoli

Osmium tetroxide, commonly used as a fixative in electron microscopy, can destroy actin filaments. Thiocarbohydrizide (TCH) is a bipolar substance that binds to the osmium. By sandwiching TCH between two phases of osmium treatment, tissue exposure to osmium could be minimized without destroying actin filaments. The contrast of osmophilic components of cells was also enhanced.

Volume 29, Issue 5, pp. 682-683, 05/01/1981
Copyright © 1981 by The Histochemical Society


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