DNA analysis and sorting of viable mouse testis cellsWM Grogan, WF Farnham and JM Sabau
The dye Hoechst 33342 and a 2-parameter cell sorter have been used to measure DNA content in viable testis cells and to sort pachytene spermatocytes and round spermatids from adult mouse testis to virtually 100% homogeneity. Early diploid spermatogenic cells were enriched to 90%, a 10-fold purification. The capability for viable sorting of most testis cell types to homogeneity in numbers suitable for many biochemical applications is demonstrated.
Volume 29,
Issue 6,
pp. 738-746,
06/01/1981
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M. Malkov, Y. Fisher, and J. Don Developmental Schedule of the Postnatal Rat Testis Determined by Flow Cytometry Biol Reprod, July 1, 1998; 59(1): 84 - 92. [Abstract] [Full Text] |
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