Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PADYKULA, H. A.
Right arrow Articles by HERMAN, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PADYKULA, H. A.
Right arrow Articles by HERMAN, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

FACTORS AFFECTING THE ACTIVITY OF ADENOSINE TRIPHOSPHATASE AND OTHER PHOSPHATASES AS MEASURED BY HISTOCHEMICAL TECHNIQUES

HELEN A. PADYKULA 1 and EDITH HERMAN 1

1 Department of Anatomy, Harvard Medical School, Boston, Massachusetts

Higher phosphatase activity toward ATP and other substrates occurred at alkaline pH in thin (5 µ), unfixed, frozen sections incubated in the medium described by Gomori ('41) than when treated with the medium employed by Maengwyn-Davies et al ('52). This difference in activities was caused principally by the salt content of the media. High sodium acetate concentration was strongly inhibitory to phosphatase activity toward adenosine triphosphate and agr, beta sodium glycerophosphate

The pH optimum of phosphatase activity of frozen sections toward adenosine triphosphate varied with the composition of the incubating medium. The localization of phosphatase activity toward adenosine triphosphate in cardiac muscle varied with substrate concentration, with nuclear staining becoming more prominent with increased adenosine triphosphate concentrations. The localization of phosphatase activity was not affected by the concentrations of agr,beta sodium glycerophosphate and adenosine-5-phosphate used, but the most rapid staining occurred with the highest concentrations.

Submitted on August 9, 1954


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Pathol.Home page
W M H Behan, D W Cossar, H A Madden, and I C McKay
Validation of a simple, rapid, and economical technique for distinguishing type 1 and 2 fibres in fixed and frozen skeletal muscle
J. Clin. Pathol., May 1, 2002; 55(5): 375 - 380.
[Abstract] [Full Text] [PDF]

Home page
 J Hand Surg Eur VolHome page
A. R. FITTON, M. S. BERRY, and A. D. McGREGOR
Preservation of Denervated Muscle Form and Function by Clenbuterol in a Rat Model of Peripheral Nerve Injury
J Hand Surg Eur Vol., August 1, 2001; 26(4): 335 - 346.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
C. T. Pu, M. T. Johnson, D. E. Forman, J. M. Hausdorff, R. Roubenoff, M. Foldvari, R. A. Fielding, and M. A. F. Singh
Randomized trial of progressive resistance training to counteract the myopathy of chronic heart failure
J Appl Physiol, June 1, 2001; 90(6): 2341 - 2350.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
W. W. Campbell, L. J. O. Joseph, S. L. Davey, D. Cyr-Campbell, R. A. Anderson, and W. J. Evans
Effects of resistance training and chromium picolinate on body composition and skeletal muscle in older men
J Appl Physiol, January 1, 1999; 86(1): 29 - 39.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
F Lang, M. Ogonowski, W. Costello, R Hill, B Roehrig, K Kent, and J Sellers
Neurotrophic influence on lobster skeletal muscle
Science, January 18, 1980; 207(4428): 325 - 327.
[Abstract] [PDF]

Home page
 ScienceHome page
B. Nystrom
Muscle-Spindle Histochemistry
Science, March 17, 1967; 155(3768): 1424 - 1426.
[Abstract] [PDF]

Home page
 ScienceHome page
L. Levine
Cytochemical Adenosine Triphosphatase of Vorticellid Myonemes
Science, May 6, 1960; 131(3410): 1377 - 1378.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1955