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Adenylate cyclase localization in unfixed specimens of rat oral mucosa and isolated mitochondria

AS Fine, RW Egnor, E Forrester and SS Stahl

The cytochemical localization of adenylate cyclase (E.C.4.6.1.1) was demonstrated in unfixed rat oral mucosal specimens and isolated mitochondrial fractions by use of a modified cytochemical reaction mixture. The adenylate cyclase reaction product was observed in the mitochondrial cristae of intact epithelial and fibroblast cells, and in isolated mitochondria after a 10-min incubation in the reaction mixture. The validity of the cytochemical adenylate cyclase method was supported by biochemical studies.

Volume 30, Issue 11, pp. 1171-1178, 11/01/1982
Copyright © 1982 by The Histochemical Society


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