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Improved mass determination of isolated biological objects by transmission electron microscopy and scanning microdensitometry

PW Linders, DJ Zahniser, AL Stols and AM Stadhouders

A modification of the classical method of Bahr and Zeitler for dry mass determination of isolated biological objects is described. The method uses a conventional transmission electron microscope (TEM) and a computer-controlled scanning microdensitometer. The performance of the modified method has been investigated by applying it to model specimens (polystyrene latex spheres). The method offers significant improvements over the existing TEM-based mass determination method.

Volume 30, Issue 7, pp. 637-644, 07/01/1982
Copyright © 1982 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1982