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A method for examining the endothelial cytoskeleton in situ using immunofluorescence

KA Rogers and VI Kalnins

A simple technique is described for producing en face preparations of endothelial cells (EC) from large blood vessels fixed in situ that are suitable for studying the distribution of specific antigens in EC by immunofluorescence. This method has permitted us to examine the distribution of various components of the cytoskeleton, including microtubules (MT), centrioles, and microfilaments (MF) in EC in vivo.

Volume 31, Issue 11, pp. 1317-1320, 11/01/1983
Copyright © 1983 by The Histochemical Society


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