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Immunogold-silver staining: new method of immunostaining with enhanced sensitivity
CS Holgate, P Jackson, PN Cowen and CC Bird
A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.
Volume 31,
Issue 7,
pp. 938-944,
07/01/1983
Copyright © 1983 by The Histochemical Society
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