Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, S. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, S. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Validity of a histochemical estrogen receptor assay. Supported by the observation of a cellular response to steroid manipulation

SH Lee

A macromolecular hydrophilic fluorescent estradiol conjugate, 17 beta- estradiol-6-carboxymethyl oxime-bovine serum albumin-fluorescein isothiocyanate, was used as a histochemical reagent to study estrogen- binding substances or putative estrogen receptors in cryostatically frozen sections of the rat uterus. Exogenous 17 beta-estradiol injected subcutaneously was found to induce or increase the synthesis of an estrogen-binding substance (or substances) in the cytoplasm of the luminal epithelial cells of the uterus in immature and adult ovariectomized female rats, whereas progesterone antagonized this effect. No effect was observed when hydrocortisone was substituted for progesterone. These intracellular responses to steroid hormone manipulation accord with the known behavior of estrogen receptors in the rat uterus. The results offer further evidence that this histochemical method is capable of identifying cell types rich in estrogen receptors.

Volume 32, Issue 3, pp. 305-310, 03/01/1984
Copyright © 1984 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1984