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Quantitative histochemical determination of muscle enzymes: biochemical verification
TP Martin, AC Vailas, JB Durivage, VR Edgerton and KR Castleman
We established quantitative histochemical assays for the enzymatic activity of succinate dehydrogenase and alpha-glycerol phosphate dehydrogenase for cat skeletal muscle. A computer-enhanced image analysis system was used to quantitate the histochemical enzyme- activity reaction products. We describe a series of experiments that verify the reliability and validity of the assays. Histochemically determined enzyme activities were linear with respect to tissue thickness and reaction time. Biochemically determined enzyme activities were also linear with respect to tissue thickness and incubation time. Consecutive tissue sections, assayed either histochemically or biochemically, were used to establish a linear regression equation that allowed quantitative histochemically determined reaction rates, measured in optical density per minute, to be calibrated as nanomoles per minute.
Volume 33,
Issue 10,
pp. 1053-1059,
10/01/1985
Copyright © 1985 by The Histochemical Society
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