Cytochemical localization of dipeptidyl peptidase II (DPP-II) in mature guinea pig spermP Talbot and G Dicarlantonio
Guinea pig sperm contain the exopeptidase dipeptidyl peptidase II (DPP- II). Our purpose was to localize this enzyme in sperm at the ultrastructural level using lysylalanyl-4-methoxy-beta-naphthylamide which is the specific synthetic substrate for DPP-II. In substrate incubated sperm, reaction product was located within the acrosome. It was concentrated over the light staining area which forms the prominent dorsal bulge of the acrosome. Within this area there are spherical zones of moderate electron density which had little or no reaction product. Control sperm did not have reaction product. We conclude that DPP-II is restricted to a compartment within the guinea pig sperm acrosome.
Volume 33,
Issue 11,
pp. 1169-1172,
11/01/1985
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K.-S. Kim, J. A. Foster, and G. L. Gerton Differential Release of Guinea Pig Sperm Acrosomal Components During Exocytosis Biol Reprod, January 1, 2001; 64(1): 148 - 156. [Abstract] [Full Text] |
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P. Syntin and G. A. Cornwall Immunolocalization of CRES (Cystatin-Related Epididymal Spermatogenic) Protein in the Acrosomes of Mouse Spermatozoa Biol Reprod, June 1, 1999; 60(6): 1542 - 1552. [Abstract] [Full Text] |
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K. Yamagata, K. Murayama, M. Okabe, K. Toshimori, T. Nakanishi, S.-i. Kashiwabara, and T. Baba Acrosin Accelerates the Dispersal of Sperm Acrosomal Proteins during Acrosome Reaction J. Biol. Chem., April 24, 1998; 273(17): 10470 - 10474. [Abstract] [Full Text] [PDF] |
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J. A. Foster, B. B. Friday, M. T. Maulit, C. Blobel, V. P. Winfrey, G. E. Olson, K.-S. Kim, and G. L. Gerton AM67, a Secretory Component of the Guinea Pig Sperm Acrosomal Matrix, Is Related to Mouse Sperm Protein sp56 and the Complement Component 4-binding Proteins J. Biol. Chem., May 9, 1997; 272(19): 12714 - 12722. [Abstract] [Full Text] [PDF] |
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S. Flaherty and N. Swann Proteases are not involved in the membrane fusion events of the lysolecithin-mediated guinea pig sperm acrosome reaction J. Cell Sci., January 1, 1993; 104(1): 163 - 172. [Abstract] [PDF] |
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